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how to produce mapping graph based on blat result?

Hi everyone,

I have a blat mapping result. The modified output format (readID, start, end, chromID, start, end) is as follows:

read1  1  98  chr1  2000  2098  +
...  ...

I also have a genome gff file as follows:

chr1  MSU_osa  exon  200  400  .  +  .  ID=LOC_Os100
...  ...

I would like to produce a mapping-graph. Previously I performed bowtie to generate .bam file that can be further used for mapping-graph production. But here how to deal with the blat output result? Thank you very much!

blat mapping

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