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Transcriptome DGE RPKM, or FPKM

Hi, all,

I am analysing the gene expression difference of a transcriptome DGE data. Two parameter RPKM and FPKM make me puzzled. I have known the exactly difference between these two words. But which one is better for me to do analysis.

Anyone please help me.

Thanks

rna-seq

If you have paired-end data, FPKM should be used.

If DGE is the goal most likely none of those should be used.

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