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Trinity -denovo transcriptome combined assembly using Illumina and 454 data

How to use trinity to perform denovo transcriptome assembly using illumina and 454 data. Please share your comments.

rna-seq
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updated by Ram Ram • written by malarvizhi.a • malarvizhi.a

you should expand your question - the simple answer is to read the manual

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updated by Ram Ram • written by Istvan Albert Istvan Albert

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Hi Istvan Albert,

Thank your suggestion. I was reading Trinity manual and found following command.

--single <string>   :single reads, one or more (note, if single file contains pairs, can use flag: --run_as_paired )

Once I have tried it. I will share my comments regarding this soon.

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updated by Ram Ram • written by malarvizhi.a • malarvizhi.a

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