Questions running Delly to detect copy number variation
Hi there. I am running delly to detect CNV.
There are 6 BAM I need to run. Is there any difference if I run it like
delly -t DUP -o sample1.out -g ref.fa sample1.bam
delly -t DUP -o sample2.out -g ref.fa sample2.bam
...
delly -t DUP -o sample6.out -g ref.fa sample6.bam
# and then merge the output vcfs
Versus
delly -t DUP -o allSamples.out -g ref.fa sample1.bam sample2.bam sample3.bam sample4.bam sample5.bam sample6.bam
Thanks
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1 answer
Second sentence of the methods section in the Delly paper:
All input libraries are analyzed jointly to achieve optimal sensitivity.
specificity > sensitivity : call separately
sensitivity > specificity : joint call
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