Hi,
I am doing scaffolding using contigs (i got these from SOAP de novo run previously) and mate-pairs (3k & 8K) using SOAP. I am preparing config file for scaffolding and i dont know what should i give for average insert size (avg_ins=?), should i give average contig size in this case, can any one help me....
1 answer
Hi
You can prepare single config file by inputting paired end reads ( used for obtaining the contigs), mate-pairs of 3k and 8k and SOAPDenovo2. You should mention the following flags in config file to decide when (rank) and for what ((asm_flags) the reads should be used .
In your case you should use as follows
For paired end reads:
asm_flags=1 & rank=1
For 3k MP:
asm_flags=2 & rank=2
For 8k MP:
asm_flags=2 & rank=3
You can follow the below link regarding insert size calculation.
Estimate Insert Size In Paired-End/Mate-Pair
I hope this helps!
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