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Tablet software to find SNPs with bam File

Hi,

I have my datas of NGS by illumina which sequenced and analyzed in bam files of tablet software but I can not understand the options and meaning of topics like: in this ,,if anybody knows to guide me ?

M00358:6:000000000-A6HCE:1:1114:8253:87 ,,,, or Cigar.....M or the BLUE and Green strap (band) shows orientation.

snp softwareerror next-gen sequencing

Hi,

I have my datas of NGS by illumina which sequenced and analyzed in bam files of tablet software but I can not understand the options and meaning of topics like: in this ,,if anybody knows to guide me?

-M00358:6:000000000-A6HCE:1:1114:8253:87 ,,,, or Cigar.....M or the BLUE and Green strap (band) shows orientation?

-What is exactly difference between read length and fragment length?

What is the coverage depth?

What is insert length?

What is properly and improperly paired?

Can you please post your question correctly ?

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