Tablet software to find SNPs with bam File

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Entering edit mode

9.5 years ago

Elnaaz ▴ 40

Hi,

I have my datas of NGS by illumina which sequenced and analyzed in bam files of tablet software but I can not understand the options and meaning of topics like: in this ,,if anybody knows to guide me ?

M00358:6:000000000-A6HCE:1:1114:8253:87 ,,,, or Cigar.....M or the BLUE and Green strap (band) shows orientation.

SNP softwareerror next-gen sequencing • 2.0k views

ADD COMMENT • link updated 3.1 years ago by Ram 43k • written 9.5 years ago by Elnaaz ▴ 40

0

Entering edit mode

Hi,

I have my datas of NGS by illumina which sequenced and analyzed in bam files of tablet software but I can not understand the options and meaning of topics like: in this ,,if anybody knows to guide me?

-M00358:6:000000000-A6HCE:1:1114:8253:87 ,,,, or Cigar.....M or the BLUE and Green strap (band) shows orientation?

-What is exactly difference between read length and fragment length?

What is the coverage depth?

What is insert length?

What is properly and improperly paired?

ADD REPLY • link updated 3.1 years ago by Ram 43k • written 9.5 years ago by Elnaaz ▴ 40