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Using Sequin, cannot put in protein translation exception.

Hello, I am attempting to use Sequin to prepare mitochondrial genome sequences for GenBank submission.

There are a handful of CDS's where the "TAA stop codon is completed by the addition of 3' A residues to the mRNA." I have left that note on all the relevant CDS's, but I am having issue inputting the translation exception to get the the "/transl_except=(pos:#..#,aa:TERM)" lines in my record viewer. I was wondering if someone could help me with this. What exactly do have I to put in the AA box to get this work? Thanks

sequin genbank mitochondrial genome
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updated by samh • samh • written by devenvyas devenvyas

2 answers

You've got to key in the amino acid number after setting a 3' coordinate on the Location tab (update your coordinate accordingly to consider the TA overhang). Your amino acid number will usually be total AA_length + 1. And, sequin will calculate the exception position for you.

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written by Hranjeev Hranjeev

I would like to add the option "/transl_except=(pos:#..#,aa:TERM)" , but I couldn't find any info how to do it. thanks

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written by samh • samh

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