MAUVE: No gene annotations in Genbank file alignments

Question

[tptacek3050]

I'm trying to align 15 genbank files generated by RAST for 15 Mycoplasma genomes that we have assembled. I am aligning the files with progressivemauve with default parameters, and visualizing with MAUVE's GUI. The alignment runs fine, but when I visualize it, I get "(no annotations loaded)" for each genome. I've tried this using a genbank file for a reference Mycoplasma genome and I get the same result.

Has anyone run into the problem before and/or does anyone have any suggestions?

Answer 1

[izwanspp]

I've had the same problem recently. It helps if you go through the Mauve_console, as it will mention what type of error has occurred during the run.

In my recent run for example, the console printed out a "locus line too short" error. So, in which case I simply edited the *.genbank file's information in the LOCUS row; shortening the identifier (the sample ID located to the immediate right of the "LOCUS" tag). I'm not sure exactly why, but perhaps a lengthy identifier messes with the tab delimited format, resulting in MAUVE being unable to read the file properly.

[yolandaly]

I just deleted the redundant gaps in the LOCUS row, then annotation loading worked!

[gidiotis]

May I kindly inquire if you could provide additional insights on how you successfully resolved this issue? I'm currently in the process of aligning two chromosomes using GenBank files that contain annotations, and I'm encountering the same challenge. Your assistance would be greatly appreciated. Thank you in advance.