Hi everyone
Could you suggest me some good tools for Gene Ontology analysis? I only know DAVID online tool, but recently saw graphical output of GO analysis in a publication (hierarchy tree structure with some terms highlighted in yellow), but don't know how to draw that. THANKS A LOT.
3 answers
Alternatively, you can use Gostats (either from bioconductor or webserver). Get the p-value of enriched GOterms and plot the p-value as barplot. If you are comparing multiple stages/celllines/tissues, make a matrix for each Goterm and p-value. Then plot it a a heatmap.
You can follow this discussion: Tools To Find Gene Ontology Term Enrichment
I personally use Cytoscape with ClueGO, STRING and DAVID. I find that these usually cover my needs. Cytoscape has a fine grain control over the images with its visual styles, and I usually end up colouring the nodes as per the fold change.
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Some of the GO analysis tools can be found here. People generally use Cytoscape and Graphviz (Cytoscape is much better) to draw GO hierarchical trees.
Thank you very much.