I am searching for methods or algorithms for the assembly process that can combine sequences from two NGS technologies (for example 454 and Illumina). Any ideas about this problem?
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Cprietos •
MIRA does hybrid assemblies. It's open source, so if the associated paper does not describe the algorithms it uses, then you can always dig into the source code to find out.
This is very much a holy grail of assembly, since the errors and depths between the two technologies differ so much.
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Jeremy Leipzig
Do they have to be aligned literally at the same time? Have you tried aligning each separately and then merging the BAM files?
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Docroberson •
Hi DocRoberson. This would be more appropriate as a comment to the original question. You should use the 'YOUR ANSWER' box only for directly answering the original question. Cheers
Thanks for the replies. I will try using MIRA.
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