I'm working on a gene discovery project and in which we've established a list of candidate genes that have mutations in evolutionarily conserved locations in subjects suffering from the disease. At this point, we want to compare structural impact of the mutations with the wild-type but many of our candidate genes do not have entries on Protopedia, let alone the mutations found in our samples.
Anyone know of some good tools for visualizing proteins based on their primary structure alone?
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sviatoslav.kendall
Did you try looking for structures in the RCSB PDB? You should only need one structure, and then evaluate the impact of the mutations based on this single structure by analysing the chemical environment of the amino acid being mutated. You can also try to do some in silico mutagenesis and evaluate the stability of the mutant by molecular dynamics for example, but for large scale study this is impractical..
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João Rodrigues
Thanks; I'll check this out. Only have 8 genes to look at so it shouldn't be too bad
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