I am blasting the following sequence to a reference on both the NCBI blastn webpage and the command line tool. However, I am not able …

I need to perform blastp having a local database of ~>1500 fasta sequences against a specific protein query sequences. So what should be the percent …

Dear all, I found a setence in one paper >The orthologs and orthogroups between bottlenose catfish and channel catfish were generated using OrthoFinder (42). To …

Hello : I am trying to run the refseq blastp command > blastp -query short_test.fa -db human_refseq -num_alignments 1 -matrix pam30 -gapopen 5 -gapextend 2 …

I am using culling_limit 1 as a parameter From the manual : Delete a hit that is enveloped by at least this many higher-scoring hits …

Hi, I'm trying to install BLAST and use it on my local machine. I've installed BLAST, but I'm not quite sure why it isn't working …

This question concerns differences between the output of blastp-short using BLAST+ vs. the web interface. When searching against RefSeq or nr (updated yesterday), BLAST+ misses …

<p>Hi,</p> <p>I have a FASTA formatted protein sequence (stored in DsbA.fa) and I would like to use PSI-BLAST (not the web server. The command line …

<p>Hi there,</p> <p>I am using blastp (using blast+ 2.2.25) to align bacterial proteins (in the file <code>multiple_queries.fasta</code>) against a custom database (<code>genome.db</code>) containing all proteins …

<p>Hi, </p> <p>I want to get top 10 sequences of BLAST results (just the sequences, no alignment or score or e-value etc). I am inputting …