Tutorial: How to predict genes using a two-pass (iterative) MAKER2 workflow

I recently found this great tutorial on how to do a two-pass annotation with MAKER and CEGMA, SNAP, Augustus, and GeneMark-ES. The repository is a treasure trove for genome assemblers.

I've tried this annotation with Swissprot-proteins and the little ESTs/RNAs I have for a 800Mbp genome using MPI and 12 CPUs and it took about 2 weeks.

Annotation tutorial

For assembly and more

assembly

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updated 3.4 years ago by Ram

• written 12.2 years ago by Philipp Bayer

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