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Recent Replies
Comment: How many reads for WGS Sequencing?
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1. What does clipped Fastq mean? both forward and reverse reads in the same file?
2. Why would you remove 2000bp contigs? I feel it will gi…
Comment: How many reads for WGS Sequencing?
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Ruqaiya
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Well, I downloaded both the reads from-[LINK][1]. Thats all i did. Thats the only thing i need to do, right?
I'l try spades.
[1]: http…
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Thanks for the fast reply.. however i had only partially understood the answer. Is correct saying that low-representation integration and b…
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Hi! Whenever you do make independent analysis or aggregate data and analyze them, most cell type assignment tools start from raw counts to …
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In my hands (and from what I know based on many years here) inputs are almost exclusively used during peak calling to correct for loci-spec…
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No difference here. After all you typically want a label per cluster/group, so either subset to the control condition to do the assignment,…
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I found the question and ASK the GPT4 turbo
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Your approach seems reasonable and well-informed, though it's perhaps less customary in the field, which could be why you haven't found pap…
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It takes 29 seconds to assemble this genome (20 CPUs) with the following statistics:
135 contigs, total 2821177 bp, min 200 bp, max …
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IMO (and unfortunately) there needs to be an effort to develop these algorithms.
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the samples that are `./.` have no coverage (or not enough to call a genotype) and the `0/0` are homozygous reference
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Did you download the complete dataset available from ENA/NCBI SRA? This is an older dataset (from 2012) with a total of 1146212 reads and 1…
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zihanss
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Hello, I want to know that why my gVCF files have "./." besides "0/0", "1/1"?
Thanks
Comment: gvcf joint calling
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zihanss
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![enter image description here][1]
[1]: /media/images/15eedc1a-b2c6-4966-be39-b5173dab
And I confused with the file that has "./." and…
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