DNA sequence in bacterial genomes
I am newbie in Bioinformatics. I have downloaded all the bacterial genomes (all.fna) from
ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/
I have some questions about the genomes and I will be appreciated if you help me to find the appropriate answer.
Are the bacterial genomes for a single strand or double strand?
In case of single strand how can I obtain the inverse strand. Should I start to read from the last letter in the genome and change the letters A --> T, C-->G, G-->C and T-->A, or its not a matter if I start from the first vs last letter in the genome?
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Devon Ryan
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raminkm2000 •
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raminkm2000 •
1 answer
The opposite strand is the reverse complement of the forward (DNA is always written 5' to 3'). So the opposite of ATGC is GCAT.
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