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Distribution of e-values in Best Reciprocal Blast Hits for Ortholog Mining

Does anyone have an idea of how the distribution of expect values will look when using reciprocal blast to detect orthologs?

blast ortholog brbh rbh

1 answer

E-values are dependent on the length of the database, so they are not really comparable between analyses. Other reasons you can't directly compare the distribution of e-values is because the duplication history and divergence level between the species being compared might vary between analyses. I would look at the distribution of Ks values, that is commonly used and it will give you something easier to interpret.

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