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Mirna Seq Blood Time Course Data Without Replicate And Paired Control

Hi,

I have question regarding analyzing time course data for miRNA -seq. I have do not have any replicates and as these samples for one conditions at different time points. As these are blood samples, so there is no paired control. Please is there any way I can generate expression profile for this kind of dataset. So far I have tested DEseq with blind method and VST specific expresssion profile. But I don't see much differences. Any suggestion would be greatly appreciated how to proceed with this data.

Thanks,

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written by raina.kumar • raina.kumar

2 answers

These are the two things I can think of:

1) Look for a significant correlation between the time point (as a continuous variable) and miRNA expression

2) See what results you can get from the 'timecourse' package

http://www.bioconductor.org/packages/release/bioc/html/timecourse.html

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written by Charles Warden Charles Warden

Thanks! I will try with time course package.

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written by raina.kumar • raina.kumar

Thanks, I will try now time course package.

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written by raina.kumar • raina.kumar

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