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How to disable FAST5 output and generate only POD5 in Nanopore?

While running a long-read sequencing experiment with Oxford Nanopore, I configured the startup settings to output POD5 files only (FAST5 generation disabled). However, FAST5 files were still generated during the run.

What could cause this behavior, and how can I enforce POD5-only output?

nanopore

Are you using the latest MinKNOW version? FAST5 files were deprecated some time back.

I'm with GenoMax here, latest versions have completely disabled fast5 output.

I have not checked, but I presumed I was using the latest version... Thank you for your help!

1 answer

Hey Kevin, I had the same thing happening last week for the first time. In previous runs this year (January) it produced only POD5, but now I got also FAST5. Maybe it is a version issue? I am using MinKNOW 25.09.16

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