I built BioSkills Lab(bioskillslab.dev), a free, self-paced bioinformatics course that runs entirely in the browser.

Course covers:

1. Unix/shell, Python (Biopython), R (ggplot2, DESeq2, Bioconductor)
2. Sequence formats (FASTA/FASTQ), QC, alignment (BWA/STAR), SAM/BAM
3. RNA-Seq differential expression, variant calling with GATK
4. Reproducible pipelines with Snakemake and Docker
5. Three end-to-end projects on real data:
6. Mystery Microbe identification — SRR24043640
7. RNA-Seq analysis: GSE52778 (dexamethasone response)
8. Variant calling: NA12878 (GIAB benchmark sample)

Also includes a Statistics for Biology course15 chapters covering distributions, p-values, multiple testing, regression, PCA, and experimental design. Based on Motulsky's Intuitive Biostatistics and ISLR.

Built-in terminal simulator supports grep, awk, cut, sort, samtools-style commands, no local setup needed to start practicing.

Everything is free, no account required.