Minimum PSM count for reliable modification stoichiometry in unenriched whole proteome open search data · Biostar

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Minimum PSM count for reliable modification stoichiometry in unenriched whole proteome open search data

What is the accepted minimum number of PSMs required to calculate reliable modification stoichiometry from open search proteomics data? Our protein of interest has 1-4 PSMs per sample. We used FragPipe with PTM-Shepherd localisation. Is this sufficient for stoichiometry reporting or is enrichment required?

proteomics ptm

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updated 4 months ago by GenoMax

• written 4 months ago by SWANITHA •

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