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Forum: Splice junctions plots for long-read seq data

Hello splicing specialists,

We have performed long-read sequencing and SUPPA2 analysis for splicing events between knock-down vs. control cells, and are looking for the best ways to visually assess the quality of our statistical tests at the individual gene level. Is there a good tool for splice junction plots or switch plots to display both transcript coverage data, together with event information (percent spliced in or delta percent spliced in) from long-read seq data? Looking around, I see mostly short-read seq data presentation that might use tools not matched to our case.

For example, panel E from this figure: https://www.science.org/cms/10.1126/scitranslmed.ade2774/asset/32891b90-b4e6-4704-9f78-ad1827e20e9e/assets/images/large/scitranslmed.ade2774-f2.jpg Coming from DOI: https://doi.org/10.1126/scitranslmed.ade2774

Shows the information we are looking to visualize. I don't see the tool for this plot in the paper.

Many thanks!

Alison

splice-junctions alternative-splicing long-read-sequencing

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