Phage whole genome sequence submission by Bankit
Hello, I am currently registering my WGS phage with NCBI/Banklt and am facing a significant issue. I have submitted the FASTA file containing all the genes, following the site's instructions, along with the feature table. Unfortunately, I am encountering an error for each gene. For instance, the sequence length for gene number 1 is reported as 507. However, when I subtract the starting point (290) from the ending point (796), I calculate the length as
- The BLAST results for each gene also indicate a
length of 507, which aligns with the site's
information. This discrepancy between the
calculated length (506) and the expected length
(507) is leading to the error. I have carefully reviewed
the gene and found no issues. Could you please
advise me on how to resolve this error without
altering the reading frame? Thank you for your
assistance.
1 answer
507 is the correct length for Seq_1. Your method of calculating sequence length (Stop - Start) is incorrect, as you undercount the number of positions in the sequence by 1. See fencepost error.
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Post an excerpt of your feature table as well.
Here is the part of the feature table
Please don't post screenshots of text data. Copy and paste the data you want to show and then format it as
codeusing the101010button.It would help to show part of the table which corresponds to the error screenshot you have shown above.
It may be best to work with NCBI help desk on this since they can access your data and can offer help. You may have already done this. Please be aware that their support may take up to 3-5 business days (MON to FRI) to get back to you but they will eventually do so. You will need to be patient.