Protoplast Transient Assay in Arabidposis
I am currently working on establishing a protocol for salt stress treatments in Arabidopsis protoplasts for transient expression assays,I would greatly appreciate your expertise on some specific aspects of the protocol.
Regarding salt stress treatments:
- What NaCl concentration do you recommend for optimal stress response in Arabidopsis protoplasts without causing excessive cell death?
- What is the optimal incubation time for salt stress treatments in your experience?
I have to incubated for 16 hr and then Add 650ul of W5 to “control” wells and 650ul of W5 (0.32M NaCl) to “stress” wells .
For protoplast transformation:
What is the optimal DNA concentration (plasmid) for PEG-mediated transformation of protoplasts in salt stress experiments?
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This is a bioinformatics site so you are unlikely to get a good answer here - it is way off topic :-)
https://biology.stackexchange.com/ might be a better place to go for wet lab questions.