Hello everyone!

I have paired microbiota data collected at two times (A and B) in which some patients responded well to treatment and others did not. I would like to do a sPLSDA on these data, predicting response based on bacterial content but using the data in as paired. I have tried multilevel sPLSDA analysis but in that case needs more than two levels! Do you have any advice? What could I do?

Thank you!