Hello (posting again because i didn't get any anwer on last post)

I have performed some genome assemblies using canu assembler and i am not able to fully understand its output files. I did find a 7/8 year old post (can't find it again) but i personally think that things might have changed since then so I am looking for current experience from you.

Organism: Fungus fusarium Data type: Nanopore ONT

After a successfull genome assembly i got the following files in output directory

• NP01.contigs.fasta
• NP01.contigs.layout.readToTig
• NP01.contigs.layout.tigInfo
• NP01.correctedReads.fasta.gz
• NP01.trimmedReads.fasta.gz
• NP01.unassembled.fasta

Looking around, i know that NP01.contigs.fasta is the main assembly file. I am not sure about the NP01.unassembled.fasta

Question 1: What is this unassembled file about. Should i consider using this file like

• polish the unassembled contigs using Racon+Raw ONT reads
• then merge them into main assembly file ?

Or just ignore this file and use the NP01.contigs.fasta for downstream processing and polishing ?

Question 2: What are the corrected reads? I mean what is a general process which generates corrected reads or how canu corrects teh reads ?

Question 3: What is the use case of readToTig and tigInfo files ?