Thanks a lot! The answer is really helpful!
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Dear all, I am running hisat2 with default settings in genomic mapping analysis. I want to run hisat2 with more strict parameters to avoid mismatched reads. Does anyone provide me some suggestions on setting relavent hisat2 options? Thank you very much!
You can adjust this parameter:
Scoring:
--mp <int>,<int> max and min penalties for mismatch; lower qual = lower penalty <6,2>
--sp <int>,<int> max and min penalties for soft-clipping; lower qual = lower penalty <2,1>
I would suggest a parameter sweep where you run multiple tests with different values to see how it affects mapping.
One consideration too is that hisat2 is soft-clipping reads, so you may want consider adjusting those options as well, You can see the scoring option above. There's also an option to disable soft-clipping entirely..
Thanks a lot! The answer is really helpful!
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