Assembly by the metaSPAes from several libraries of paired-end reads with different reads lengths.
Dear forum members. Could you clarify please.
I have two libraries: 2x300 Illumina and 2x150 MGI. These are metagenomic data from different locations in the lake. I want to assemble them into one assembly by metaSPAdes (cross-assembly).
cat R1_300.fq.gz R1_150.fq.gz > combine_R1.fq.gz
cat R2_300.fq.gz R2_150.fq.gz > combine_R2.fq.gz
Is it possible to do this? Maybe there are specialized programs for combining, so that there are no problems with insert size. metaSPAdes, judging from the manual, support only one library paired-end reads, so the --dataset option will not work. I came across a doubt that reads can be combined using the cat command. How to do it in this situation?
• 652 views
•
link
0 answers
No answers yet.
Log in to answer this question.