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How to calculate the P-distance in Heteroplasmy Analysis Using mtDNA PacBio Data: Tools and Procedures

How to calculate the P-distance in heteroplasmy analysis using mtDNA PacBio data. I have already identified somatic mutations in heteroplasmy analysis. Could you suggest tools or a procedure for this?I humbly request your guidance from the beginning. Could please someone help me?

Thank you for your assistance.

pacbio heteroplasmy somatic-mutations mtdna

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