Hi everyone! For my thesis, I sequenced the whole-genomes of bacteria of the Rhodobacterales order. I performed genome annotation with RASTtk, but this tool only uses annotation data in the SPEED database to recognize the presence of certain genes. Now I wonder, if you work with bacteria whose sequences are not present in the database, if the annotation's quality is still good. Are there any alternatives for genome annotation that do not make use of specific databases? Thank you in advance!