I have an output from MIXCR that contains TCR V,D,J,C regions for both the alpha and beta chains, respectively. However, I want to pair them to fold the TCR for future experiments. We have discussed switching to a sc-approach but curious if anyone has figured out this pairing from a bulk approach.

We tried frequency matching (most common alpha + most common beta is a pair, etc) but this has not worked. We did get the V,D,J sequences from uniprot rather than the imputed ones from MiXCR but after careful examination, I am not confident that is the issue.

Thank you!