Failed kmer content
I have an Illumina sequencing data . When I ran fastqc it did not show any column for kmer content. When I cleaned the data using 50bp as minimum length and q20 as the phred quality score , the fastqc report had an added column for failed kmer content . Why is it showing & how to solve this.?
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Can you post a screenshot of what you are referring to? Why did you choose to "clean" your data?
FastQC analysis needs to be done keeping the context of the experiment in mind. Getting all "green checkmarks" is not the end result desired.