Hello,

I have a naive question about using Velocyto on 3' single-cell data.

My doubt is about the minimal read length that is required to obtain useful information from the data: Velocyto relies on spliced and unspliced reads ratio, and I am afraid that single-end reads, if too short, would not be enough for obtaining this information and consequently the RNA velocity.

Do you have any kind of suggestions or experience on that?

Thanks a lot.