miseq double band
I am in the process of preparing an Illumina MiSeq library for 16S analysis. However, I'm encountering double bands in some samples during adapter PCR, and in all samples during index PCR. These samples are from monkey fecal material, and interestingly, no bands are appearing in the negative controls. Even after reducing the extension time, the double bands persist, with a size of 1500bp. Can you please help me understand the reason behind this?
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junghwachoi96 •
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