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Illumina NextSeq 500 Read Length

Hi scientists,

I have downloaded secondary dataset from NCBI GEO Dataset sequenced through Illumina NextSeq 500. QC report of the data tells that read length ranges between 10-76. Is it normal?

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ngs read nextseq
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updated by jv jv • written by Bushra Bibi Bushra Bibi

10 bases is pretty short for sequencing data, depending on the type of data and your goals you might benefit from additionally filtering for a min read length if you plan to use this data

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written by jv jv

Thanks jv!

My goal is to perform differential gene expression analysis. What should be the minimum read length?

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written by Bushra Bibi Bushra Bibi

1 answer

QC report of the data tells that read length ranges between 10-76. Is it normal?

If the data is pre-trimmed then yes this can be expected. If you know that the data is not trimmed then every read should be the same lenght (max number of cycles sequenced).

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written by GenoMax GenoMax

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