How to make separate plots in Monocle3 using combined control and treatment samples
Dear experienced users,
Do you know how to make seperate plots in Monocle3 using combined control and treatment samples?
The group_cells_by = "partition" seems not works even though the partitions (cds_combined. show there are 2 levels); I use load_cellranger_data() to get the cds1 and cds2, and combine use combine_cds(list(cds1, cds2))
Thank you very much!
EDIT:
Here's the full code:
cds1 <- load_cellranger_data("~/S1")
cds2 <- load_cellranger_data("~/S2/")
cds_combined <- combine_cds(list(cds1, cds2))
cds_combined <- preprocess_cds(cds_combined, num_dim = 100)
cds_combined <- reduce_dimension(cds_combined)
cds_combined <- cluster_cells(cds_combined, num_dim = 100)
cds_combined <- learn_graph(cds_combined)
plot_cells(cds_combined, genes =c ("EGFR"), group_cells_by="partition", color_cells_by="partition")
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