I want to annotate cell type of an snRNA-seq dataset of brain tissues(motor and frontal cortex)
I have metadata file and another file which categorizes genes into ~12 cell types.
Can I copy annotations from metadata file for common cell barcode? I used Kallisto-bustools for count matrix and CellRanger was used by the original authors. There is about 50-60% of cells with common barcode.
From the second file which has cell categorized, can I manually annotate by querying this file with results from findmarkers of each cluster? Importing the dataset into R remove the cell types and only imports the 11 column and genes as rows.
Any other method which will work here?
Thanks for your time.
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myoui3122010 •
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