Explanation of definition lines for Trinity .fasta and .SuperTrans.fasta files
Hi folks,
I assembled a transcriptome in Trinity v2.8.5 using the --include_supertranscripts parameter. These are the deflines for the .fasta file:
>TRINITY_DN8_c3_g1_i1 len=330 path=[0:0-329]
>TRINITY_DN8_c1_g1_i1 len=271 path=[0:0-270]
>TRINITY_DN8_c2_g1_i1 len=357 path=[0:0-356]
>TRINITY_DN8_c0_g1_i4 len=2132 path=[0:0-1596 2:1597-1673 3:1674-1734 4:1735-1789 8:1790-1797 9:1798-1927 11:1928-2025 12:2026-2066 13:2067-2096 15:2097-2131]
These are the deflines for the .SuperTrans.fasta file:
>TRINITY_DN8_c1_g1
>TRINITY_DN8_c0_g1
>TRINITY_DN8_c0_g2
>TRINITY_DN8_c0_g3
>TRINITY_DN10_c1_g1
>TRINITY_DN10_c2_g1
To my understanding, g1 indicates gene 1, i1 indicates isoform 1 of gene 1, len is the length of the transcript and path indicates the nodes of the de Buijn graph transversed by the transcript. What is indicated by DN8, D10 and c0, c1, c2, etc? Ive been searching around but cannot find an explanation. Thank you!
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Per ChatGPT
Let's break down the components of this header:
For Supertranscript headers :
Length Information (len=1200):
Supertranscript Indicator (super=1):
Path Information (path=[1234:0-547 567:200-899]):