Psuedobulk analysis of single cell RNA-Seq data
I have a single cell RNA Seq dataset from Fluidigm C1 Chip. Given that I have 50+ and 30+ cells in two groups respectively, would it be okay, to merge the Fastq files of each groups and do a pseudo-bulk analysis ?
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Nitin Narwade
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evocanres •
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evocanres •
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If I understand you right, this would turn your data into
N=1for each group. Possible to do a hacky DGE but not a well designed one.trivial as it may sound, how about making N=3 for each group ?
Actually, you can, by random subsampling.
Nice tutorial from Fabian Theis could be of great help.
Cheers!!
Nitin N.