KEGG ribosomal gene found in metatranscriptome despite removing rRNA sequences
I am conducting metatranscriptome analysis of human feces. I used SortMeRNA to remove rRNA sequences before assembly and then get predict gene sequences from the contigs. However, when I obtain KEGG KO annotations for the predicted gene sequences using diamond blastp, not a few sequences were associated with "ribosome". Additionally, there are many reads mapped to these sequences, resulting in a high representation of ribosome genes in the "gene composition" of the metatranscriptomes.
Is this occurs commonly? Should I consider excluding predicted genes with "Ribosome" when I get gene compositions as well as rRNA sequence removal by sortmerna?
Thanks.
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