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Deseq2 error

Hello everyone,

I was performing deseq2 using R and I came across the following error. I have downloaded the R scripts and deseq2 as mentioned in the biostar sub-book RNAseq by Example.

cat simple_counts.txt | Rscript deseq2.r 2x2 > results.txt
dyld: Library not loaded: @rpath/libz.1.dylib
  Referenced from: /Users/sgadila/micromamba/envs/bioinfo/lib/R/lib/libR.dylib
  Reason: image not found
Abort trap: 6
(bioinfo)

Could anyone please help me with this issue? Also, could you please explain if this is an issue with the deseq2 or R?

r deseq2

What is this deseq2.r - where did you get it from and what are is contents?

Thanks for your response. I was using Biostar handbook and looks like this is from the old version of the book.

Please edit your post and mention this there. Also paste the contents of deseq2.r in there.

deseq2.r code is now in a sub-book called RNAseq by Example which is part of biostar handbook. Are you using the instructions in that book?

Yes, I did set up my computer as mentioned in the sub-book RNAseq by Example and also downloaded the RNAseq scripts from the book to perform the analysis.

Can you please edit your post and add the information I asked you to add?

Sorry, I just edited my post.

What version of macOS are you using and did you install a different python than what came with your macOS.

I am using version 10.13.6 of macOS and yes, I have installed Python 3.8.18.

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