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RNA-Seq

I have a strand specif RNA-Seq data with strand-specific RNA-Seq bam files. Now, I want to map positive and negative gene locations in gtf file, on bam file. So, should I map positive locations on reverse bam and vice versa?

rna-seq

What are you actually trying to achieve with your data? Why do you have already mapped data that you now want to map a second time? Have you searched the forum (i.e. this discussion might be helpful?)?

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