how to solve the problem "Invalid character (+) in sequence"
When I annotated the metagenomic data with args_oap_3.2.3, the following error was reported.
I found the contents of the line in the corresponding file as follows. I don't understand what the problem is, how to solve it.
• 2,286 views
•
link
written
by
alice005201314 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
why I get "Running twoBitToFa failed" when loading chopchop
written by WYZ1120zzh •
-
Understanding IGV
written by zizigolu
-
Increasing per base G content in QC sequencing files
written by robertsr •
-
why 99% reads failed to align in mir-seq analysis?
written by mohammadhassanj
-
Getting sequence information by using atoms
written by drbioengineer •
-
Why my `Percent duplicates` in a shallow sequencing is too high?
written by 631079064 •
-
Is the current R version (4.1.2) are not suitable for the packages related with TCGA
written by nonaddldy •
-
Sequence Duplication Levels
written by Elizaveta •
-
Extremely high adapter content and low alignment rate in RNAseq
written by ngs101.com
-
Tutorial: Extracting genomic feature sequences from GTF/GFF files with AGAT
written by Juke34
Your fastq file is broken, the line after
@SRR10492803.32660369 32ACATTTT...is+, this line should be sequences line, not+. You need to use md5sum to check md5 files after downloading data.Thank you. I thought that might be the reason
The program expects a fasta file but you're giving it a fastq file?
The fastq files are also available