Hi, I am using Kallisto and Sleuth to analyze my RNA-seq data. I have an issue performing a step to associate transcripts to genes using the command "aggregation_colum = 'ens_gene'. I received an error message:
"Error in check_target_mapping(tmp_names, target_mapping, !is.null(aggregation_column)) : There is at least one duplicated target ID in the target mapping. Since sleuth prep is in gene aggregation mode, any duplicated entries will cause errors during gene aggregation."
However, I didn't find any duplicated target ID from the abundance tsv file generated from Kallisto. Can you please help me to figure out the possible cause of this error? I would highly appreciate your help.
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