Hello everyone,

I have my annotated seurat object and I want to perform Nichenet analysis. I tried to follow this tutorial but I stacked at the second step (define the gene set of the interest) since I do not have any aggregation dividing. I only have several cell types (cancer, b cells, t cells, stem, and plasma).

I would like to understand of not only ligand-receptor communication but also the response of each communication type.

How should I perform such analysis?

Thanks in advance