Thank you so much!!
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Hi,
I'm doing cell-cell interaction anlaysis with Cellchat package in R. To identify over-expressed signaling genes, I want to set the thresh.fc argument of OverExpressedGenes() function.
I have a question about this thresh.fc argument.
Documentation says it's Log fold change threshold. those that mean Log2 fold-change? or Log10 fold-change?
Thank you
It seems they are taking the natural log of the mean expression and calculating the difference.
mean.fxn <- function(x) {
return(log(x = mean(x = expm1(x = x)) + 1))
}
data.1 <- apply(X = data.use[features, cell.use1, drop = FALSE],MARGIN = 1,FUN = mean.fxn)
data.2 <- apply(X = data.use[features, cell.use2, drop = FALSE],MARGIN = 1,FUN = mean.fxn)
FC <- (data.1 - data.2)
You can have a look at the code here.
Thank you so much!!
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