Dear guys,
May I have your guidance that what the conclusion can you get from the cell cycle analysis of scRNAseq data?
Can I say the cells near 1/2 or partial cells are going through the proliferation? Is there a way to get the ratio of the cell numbers of G1, S, G2M? Why G2M, not seperated G2 and M?
Thanks a lot!
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written
by
alwayshope •
2 answers
G2/M can be hard to separate, and the genes you might use to separe them could give unreliable results. By counting cells annotated as S, G1, or G2/M you can get the ratios. cells near the G1 group may be early S or late G2M, while further separated cells could be later S, earlier G2M. However, there are always caveats. In terms of proliferation, I'm not sure you could say a lot since you don't know if G1 cells are arrested or preparing for S phase. If the cell is in G1 but preparing for S, I would still consider it proliferating.
Totally agree with rfran010. The cell cycle analysis does not quite make sense. I'd rather compute the cycling score and see which cell (or cluster) has the highest score.
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