ComBat returns negative expression value
Hi, I am trying to use ComBat function to remove the batch effect:
combat_data <- ComBat(dat = trans_log, batch = colData$batch)
But it returns some negative value, I'm not sure if this is because the batch effect itself is not obvious?
Can I use the negative value to do WGCNA analysis, or I don't need to do ComBat? (Because my batch effect is really not obvious)
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