This is a test version of Biostars. For the public version, visit https://www.biostars.org.
RNA STAR Solo, to many cells?

Hi guys,

I am trying to map online scRNASeq Data to the murine genome using STAR (Galaxy Version). Looking at my results I get the following output:

Barcodes:
                                       noNoAdapter              0
                                           noNoUMI              0
                                            noNoCB              0
                                           noNinCB              0
                                          noNinUMI              0
                                  noUMIhomopolymer              0
                                       noNoWLmatch              0
                                       noTooManyMM              0
                                noTooManyWLmatches              0
                                   yesWLmatchExact              0
                               yesOneWLmatchWithMM              0
                              yesMultWLmatchWithMM              0
Genes:
                                        noUnmapped       34353576
                                       noNoFeature       49132949
                                      MultiFeature       35781538
                       subMultiFeatureMultiGenomic       31881499
                                noTooManyWLmatches              0
                              noMMtoWLwithoutExact              0
                                        yesWLmatch      201362414
                                yessubWLmatchExact      201362414
                       yessubWLmatch_UniqueFeature      201362414
                                   yesCellBarcodes        1561374
                                           yesUMIs      125622644

Does anybody have an Idea why I get so many cells? As I understand it correctly yesCellBarcodes means the number of cells which were detected but 1561374 seems way to high...

star

0 answers

No answers yet.

Log in to answer this question.