I am working with total counts of gene sequences, not relative abundances and want to do a PCoA and Adonis. Do I have to normalise, log transform or otherwise prepare my data beforehand?
Dear everyone, I have a dataset of absolute counts and relative abundance obtained from qPCR experiments. I'm interested in calculating the Bray-Curtis dissimilarity to analyze …
Hi, I have biological replicates: they come from the same raw sample (tube of sediment) but from different DNA extraction & sequencing experiments. These are …
In metagenomics datasets, it is standard practice to correct samples for (a) differences in sequencing effort (library size) and (b) normalise gene counts based on …