Normalized counts bulk RNA-seq to DEGs
Hi,
I have a RNA-seq count matrix and normalized by DESeq2. Unfortunately, I need to improve a workflow to find DEGs from the normalized count. I already checked edgeR, DESeq2 and limma. However, they required un-normalized count matrix. Do you have any suggestion? Thanks.
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I do not understand your question, you said you started with a count matrix that you normalized with DESeq2. Why don't you follow the DESeq2 workflow until the end ?
I only have output as normalized counts. I did not do that part.
Then ask for raw counts. Analysis is anyway not reproducible and hence not publishable without the raw data at hand.
Follow up on DESeq2 for different design and normalized counts and your many previous questions from which many received no upvotes or accepts.